The SpinChip technology is based on the unique dual axel centrifugation of the assay cartridges combined with patented assaying and ultrasensitive features. The SpinChip instrument is further holding two complementary optical readout systems. This multidisciplinary combination allows for extreme efficiency, flexibility and robustness in sample and reagents processing as well as measurement over a very wide sensitivity range.
A small sample (typically 1µL - 15µL whole blood) is drawn directly into the sampling device integrated within the cartridge. The sampling device may be modified to draw a variety of samples and sample volumes including semisolid and tissue based samples.
Dual axel centrifugation
Upon feeding the closed assay cartridge into the instrument, it is automatically placed and locked off centre in the rotor disc. The processing and readout of the cartridge occurs automatically in accordance with the QR code printed on the cartridge.
The uniqueness of the SpinChip dual axel concept is that the orientation of the cartridge relative the centrifugal force is altered bi-directionally while spinning. The orientation of the cartridge is at any time monitored and controlled by the vision system securing high accuracy and reproducibility in the cartridge processing.
The SpinChip cartridge holds integrated both liquid and dried reagents. The microfluidic designs are specifically designed to process the sample and the reagents according to the assaying and readout principles used. These designs consist of a variety of microfluidic modules connected by channels in several layers within the cartridge.
When centrifugal force is applied to the cartridge, liquids (sample and reagents) will flow through available paths to the cavity within the cartridge that at any time is farthest from the centre of the rotor disc. A variety of microfluidic actions are applied to the liquids by the act of controlled alterations of the cartridge orientation relative to the centrifugal force combined with unique designs of channels and cavities within the cartridge.
Liquid processing includes dissolving dried reagents, mixing, splitting, exact metering, dispensing, flow through filters, stacking and dispersing of beads, flow through stacked columns of beads as well as centrifugal separation of plasma from blood cells and beads from any liquid.
The various liquid processing modules are combined in designing the complete integrated assays in accordance with the specific requirements of the samples, the preferred analytical assaying sequence and specific readout features as desired. SpinChip has established state of the art rapid prototyping of cartridges in house.
The SpinChip instrument includes state of the art mechanics, mechatronics, electronics, optics, user interfaces, connectivity features and embedded software for fully automatic operation of the cartridge, optical readout and appurtenant processing and communication of information and results. The operator may communicate with the instrument through the touch display or through the connectivity system.
Two complementary optical readout systems are placed along the spinning path of the of rotor disc.
- The spectral readout system plays a key role in real time control of the cartridges with respect to orientation and content. This readout system is further used for reading color intensities from surfaces by reflectance readout and optical densities of liquids by transmittance readout.
- The genuine fluorescent readout system allowing for high sensitivity measurements.
The combination of two complementary readout makes the platform cover a wide sensitivity range and broad flexibility in the use of assaying readout principles.
SpinChip is presently focusing on two different demonstrator concepts based on immunometric assays. The low sensitivity concept for the measurement of CRP and a high sensitivity concept for the measurement of high sensitivity cardiac Troponin I (cTnI).
The SpinChip competences and facilities covers state-of-the art immunochemistry, functionalization of membranes, beads and nanoparticles as well as stabilization and preservation of reagents and immunometric assay designs.
A key to success is know how as to the functionalization and formulation of reagents, including the formulation of these reagents as precise and durable dried spheres.